NASC

NASC ID: N2922

Name: Meinke and Feldmann - 4851

ABRC stock number: CS2922

Description: Form

Donation Date: 1992-04-30

Donated by:

Oklahoma State University David Meinke University of Arizona Ken Feldmann

Donor Number: 4851 -

Stock type: individual line

Material type: seed

Comment: This line has now been replaced by N3867

Status	Price (£)
Retired	£11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Ws-2(N2360)

Growth requirement: none

Generation: T4

Segregation: Yes

Transformation method: Agrobacterium transformation

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
	lec1-2	lec1	At1g21970

Phenotype

embryo lethal; viviparous embryos with cotyledons partially transformed into leaves; embryos sensitive to abscisic acid; embryos have reduced hypocotyl, cotyledons that remain green late in development, and reduced protein and lipid bodies; some trichomes form on cotyledons after germination; internal cells of cotyledons enlarge, vacuolate and resemble leaf mesophyll cells; intercellular spaces are prominant in cotyledons; enlarged shoot apical meristem; stomata are found on surface of cotyledons of mutant embryos; mature seeds are desiccation intolerant; root apex is active in viviparous seeds; immature mutant embryos germinated prior to desiccation produce plants that appear normal for other characteristics; maintained as heterozygote.

References

Meinke, D.W. et al. 1994. Leafy Cotyledon Mutants of Arabidopsis. The Plant Cell 6(8):1049-1064.PubMed ID: 0012244265.